DNA

Part:BBa_K4781009

Designed by: Avijit Majumder   Group: iGEM23_IISER-Kolkata   (2023-10-11)


crRNA-fliC-direct repeat

crRNA-fliC-direct repeat will produce guide RNA against the flagellin protein Xanthomonas oryzae pv. oryzae with a direct repeat at the end which can be used to make crRNA array . This 19 nucleotides direct repeat is an essential part of the guide RNA of FnCas12a. To design guide RNA, 20nt complementary sequence of the target gene just has to be added after the direct repeat.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None